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Hello. I apologize that no one responded to you sooner. Different experts come on at various times. I just logged on and saw your question. My name is ***** ***** I'm a biologist with a special interest in fish. I'm sorry to hear of this problem. Some additional information will help me figure out what is happening. How are the koi behaving - gasping at the surface, swimming sideways, floating, hanging out by the waterfall, etc? Did you use any kind of algae removing chemical? If so, what? Was the water you filled the pond with de-chlorinated? Thank you.