I am an independent computer expert from South Africa here to assist.
If you already looked inside 'Recycle bin' and do not see it there, then we can assume that the file was permanently deleted.
The best way would be to run recovery software that can scan the computer for deleted files, and help you to undelete them.http://www.runtime.org/
has recovery software that will work on Windows Vista. Please click on the 'NTFS' link under the 'GetDataBack' heading to download it.
Here is a direct link.http://www.runtime.org/gdbnt.zip
It is best not to save the file to the save drive where you stored the Ladybug.pub file, as any new files created on the hard drive will use the space used by the deleted files, removing any possibility of recovering it. If you have another hard drive, then install it to that drive. You should also be able to install it to a memory stick, or other removable drive.
Then start the program when the installation completes, and do a scan.
It will list all the recently deleted files.
Let me know how that goes.