General

Ask Experts & get answers to your questions - ASAP

Ask an Expert,
Get an Answer ASAP!

I'm trying to find out the properties of the microbes E., S.

Customer Question
I'm trying to find...

I'm trying to find out the properties of the microbes E. coli, S. epidermidis and S. cerevisiae.

Expert's Assistant: The expert will know what to do. Please tell me everything you can so the expert can help you best.

I identified E. coli as a gram negative, mobile bacteria with catalase activity that grows best in a warm, dark, enviornment. I was told my discussion of characteristics associated with the VP were not evident and an appropriate description of the characteristics of properties was not complete,.

Expert's Assistant: Is there anything else the expert should be aware of?

Escherichia coli Reagents (if required) Observation of results Interpretation of resultsMethyl Red Voges-Proskauer Catalase Motility These are the questions I was supposed to answer. I believe that nothing with my S. epidermidis yielded the right results.Staphylococcus epidermidis Reagents (if required) Observation of results Interpretation of resultsMethyl Red Voges-Proskauer Catalase Carbohydrate Fermentation- Fructose Carbohydrate Fermentation- Glucose Carbohydrate Fermentation- Mannitol Motility Salt Tolerance:Nutrient broth with 1% NaCl Salt Tolerance:Nutrient broth with 7% NaCl Salt Tolerance:Nutrient broth with 15% NaCl Saccharomyces cerevisiae Reagents (if required) Observation of results Interpretation of resultsCarbohydrate Fermentation- Fructose Carbohydrate Fermentation- Glucose Carbohydrate Fermentation- Mannitol Salt Tolerance:Nutrient broth with 1% NaCl Salt Tolerance:Nutrient broth with 7% NaCl Salt Tolerance:Nutrient broth with 15% NaCl

Submitted: 2 years ago.Category: General
Show More
Show Less
Ask Your Own General Question
Answered in 23 hours by:
2/16/2016
Expert: JACUSTOMER-lesqa7tl-,
 replied 2 years ago
JACUSTOMER-lesqa7tl-
Category: General
Satisfied Customers: 39
Verified

I have another commitment right now and will give you a full reply later today. Sorry for the inconvience

Ask Your Own General Question
Customer reply replied 2 years ago

Thank you for your response. Later today will be great. Is what I need help with clear from the information i have given you?

Expert: JACUSTOMER-lesqa7tl-,
 replied 2 years ago

Let me know if this answers your questions.
I've never tried growing the organisms at 15% NaCl, so have no direct experience with that concentration, but the literature seems to point to the results I've outlined

Even pure strains of the bacteria can have genetic mutations that allow them to tolerate different saline concentrations or ferment unusual compounds, nothing is 100% in microbiology, but that is the fun part.

====================================================

VP Test
Add potassium Hydroxide + creatine (KOH) & alpha-naphthol,
Shake vigorously, let stand ~ 5-10 minutes, some references indicate1-2 hours, exposed to air.

Negative (-) = light brown color, (E. coli), Positive is a pink color (Enterobacter)

MR-VP, usually run at the same time, Record the MR results (red+, yellow -) before adding the VP reagents

The corresponding Methyl Red test is Positive for E.coli (red) and Negative for Enterobacter

Staphylococcus epidermidis
Fructose Positive (Ferments)
Glucose Positive (Ferments)
Mannitol Negative (Staph aureus is Positive)
Motility Non Motile (Staph are cocci (round) and are not motile
Salt Tolerance 1% Growth
Salt Tolerance 7% Growth
Salt Tolerance 15% slow or no growth

Saccharomyces cerevisiae
Fructose Positive (Ferment)
Glucose Positive (Ferment) (prefers Glucose over Fructose)
Salt Tolerance 1% Growth
Salt Tolerance 7% Inhibition or No Growth
Salt Tolerance 15% No Growth

Ask Your Own General Question
Customer reply replied 2 years ago
Just one thing I wasn't clear on. With S. cerevisiae, is carbohydrate fermentation mannitol positive?
Expert: JACUSTOMER-lesqa7tl-,
 replied 2 years ago

It appears that Saccharomyces cerevisiae cannot ferment Mannitol, although some wild strains can and the yeast can be adapted to the sugar

Ask Your Own General Question
Customer reply replied 2 years ago
Thank you so much. You have answered all my questions.
Was this answer helpful?

How JustAnswer works

step-image
Describe your issueThe assistant will guide you
step-image
Chat 1:1 with an expertLicensed Experts are available 24/7
step-image
100% satisfaction guaranteeGet all the answers you need
Ask Ellen Your Own Question
Ellen
Ellen
Ellen, Consultant
Category: General
Satisfied Customers: 109
109 Satisfied Customers
Experience: attorney and legal researcher

Ellen is online now

A new question is answered every 9 seconds

How JustAnswer works:

  • Ask an ExpertExperts are full of valuable knowledge and are ready to help with any question. Credentials confirmed by a Fortune 500 verification firm.
  • Get a Professional AnswerVia email, text message, or notification as you wait on our site. Ask follow up questions if you need to.
  • 100% Satisfaction GuaranteeRate the answer you receive.

JustAnswer in the News:

Ask-a-doc Web sites: If you've got a quick question, you can try to get an answer from sites that say they have various specialists on hand to give quick answers... Justanswer.com.
JustAnswer.com...has seen a spike since October in legal questions from readers about layoffs, unemployment and severance.
Web sites like justanswer.com/legal
...leave nothing to chance.
Traffic on JustAnswer rose 14 percent...and had nearly 400,000 page views in 30 days...inquiries related to stress, high blood pressure, drinking and heart pain jumped 33 percent.
Tory Johnson, GMA Workplace Contributor, discusses work-from-home jobs, such as JustAnswer in which verified Experts answer people’s questions.
I will tell you that...the things you have to go through to be an Expert are quite rigorous.

What Customers are Saying:

Wonderful service, prompt, efficient, and accurate. Couldn't have asked for more. I cannot thank you enough for your help.

Mary C.Freshfield, Liverpool, UK

This expert is wonderful. They truly know what they are talking about, and they actually care about you. They really helped put my nerves at ease. Thank you so much!!!!

AlexLos Angeles, CA

Thank you for all your help. It is nice to know that this service is here for people like myself, who need answers fast and are not sure who to consult.

GPHesperia, CA

I couldn't be more satisfied! This is the site I will always come to when I need a second opinion.

JustinKernersville, NC

Just let me say that this encounter has been entirely professional and most helpful. I liked that I could ask additional questions and get answered in a very short turn around.

EstherWoodstock, NY

Thank you so much for taking your time and knowledge to support my concerns. Not only did you answer my questions, you even took it a step further with replying with more pertinent information I needed to know.

RobinElkton, Maryland

He answered my question promptly and gave me accurate, detailed information. If all of your experts are half as good, you have a great thing going here.

DianeDallas, TX

< Previous | Next >

Meet the Experts:

Ellen

Ellen

Consultant

109 satisfied customers

attorney and legal researcher

P. Simmons

P. Simmons

Attorney

149 satisfied customers

45 years old, retired Marine Officer, current attorney

Kim D.

Kim D.

Consultant

34 satisfied customers

Researcher 25 yrs in small and large institutions

SuperiorConsultant

SuperiorConsultant

Internet Researcher

21 satisfied customers

BBA, serving as Marketing Executive and interested in tourism,sports, and anything and everything!

Michael

Michael

Librarian

1,903 satisfied customers

20+ years as information professional

Cher

Cher

Educator-40+ yrs

1,702 satisfied customers

M.A., B.A., Author, Information & Research Specialist

Martin

Martin

Engineer

886 satisfied customers

i'm 41 and i never stopped studying and experimenting

< Previous | Next >

Related General Questions
Is everything more expensive in California? How much more
Is everything more expensive in California? How much more expensive are food and hotels in LA or San Francisco compared to TN? If someone does not go to a big city, are stuff still more expensive?… read more
Cher
Cher
Educator-40+ yrs
Masters Degree
1,702 satisfied customers
I got my transcripts from Kentucky but it does not show my
Hi Allen my name is***** got my transcripts from Kentucky but it does not show my date of graduation and my prospective employees looking for that. Can you help me in acquiring that information I lost… read more
Michael
Michael
Librarian
Bachelor's Degree
1,903 satisfied customers
Could you please explain me in details what does it mean
could you please explain me in details what does it mean "quit thinking that everyone is out to get you"? Unfortunately I'm Italian and strive to improve American English. Thank you. GIULIANO… read more
Michael
Michael
Librarian
Bachelor's Degree
1,903 satisfied customers
I am trying to track a package which was received In at ISC
I AM FROM Barbados. I am trying to track a package which was received In at ISC New York NY on May 08 2017. The tracking #R!200695087BB… read more
Rarewares
Rarewares
Expert Appraiser/Antiques Dealer
659 satisfied customers
If the Bahamas, the Dominican Republic, and Haiti are
If the Bahamas, the Dominican Republic, and Haiti are considered to be part of North America, why is it taught that Christopher Columbus didn't land on North America in 1492? Thank you.… read more
Michael
Michael
Librarian
Bachelor's Degree
1,903 satisfied customers
What's the process like to sell on amazon? Is it a lengthy
What's the process like to sell on amazon? Is it a lengthy process to sign up. I own a Retail clothing store so I would be selling clothes, jewelry and accessories. Thx… read more
Michael
Michael
Librarian
Bachelor's Degree
1,903 satisfied customers
I rent my apartment on sites like Airbnb and Booking and
Hello, I rent my apartment on sites like Airbnb and Booking and would like to know what I could offer to please the guest, in case something is not what he/she expected in the apartment (Eg. chocolate… read more
Michael
Michael
Librarian
Bachelor's Degree
1,903 satisfied customers
Dear Education Expert, I have heard that there are some
Dear Education Expert,I have heard that there are some universities in the US that I can get a PHD on my inventions/existing educations.How can I find them ?Many thanks… read more
jennem22
jennem22
Appraiser
Bachelor's Degree
57 satisfied customers
Where can I submit press releases where it's likely that
Where can I submit press releases where it's likely that they will be featured on the major sites like Forbes or Entrepreneur? I know there are people who know how to "hack" this and I'd like to find … read more
Michael
Michael
Librarian
Bachelor's Degree
1,903 satisfied customers
I'm having trouble finding square halo yellow gold earring
I'm having trouble finding square halo yellow gold earring jackets for a 1 carat princess cut earring. Can someone help me find it?… read more
Nora
Nora
Accredited Appraiser & Antique Expert
Post-Graduate from Christies London
174 satisfied customers
Are there any stores in Nashville and surrounding areas that
Are there any stores in Nashville and surrounding areas that sell foldable wheelchairs? Thanks.… read more
Schuyler
Schuyler
1,944 satisfied customers
What caused many people to lose their jobs in Flint,
What caused many people to lose their jobs in Flint, Michigan on or around the 1980s and become homeless and/or poor?… read more
Steve
Steve
I&C technician
Vocational, Technical or Trade Scho
7,185 satisfied customers
Was the apostle a false prophet? was cut off from the church
Was the apostle Paul a false prophet? Paul was cut off from the church in Jerusalem because the doctrine that he was preaching contradicted what they were teaching. And the critical problem is that Ja… read more
Michael
Michael
Librarian
Bachelor's Degree
1,903 satisfied customers
I live in the UK and own number 6 of 25 edition 70cm high
I live in the UK and own number 6 of 25 edition 70cm high Nicky Imber bronze sculptures titled "Hope". I want to insure the sculpture for its current value on the open market. Is the current value dif… read more
Rarewares
Rarewares
Expert Appraiser/Antiques Dealer
659 satisfied customers
If i did a lab using phenol red testing of fructose,
If i did a lab using phenol red testing for fermentation of fructose, glucose, and mannitol. And i tested staph epidermidis ans s cerevisiae. What would be the fermentation profile of each. And what c… read more
JACUSTOMER-lesqa7tl-
JACUSTOMER-lesqa7tl-
39 satisfied customers
I am doing carbohydrate fermentation testing on organism saccharomyces
I am doing carbohydrate fermentation testing on organism saccharomyces cerevisiae and E. Coli,we used the phenol red fermentation tubes. the yeast came out profile glucose AG, fructose A, Mannitol A,w… read more
JACUSTOMER-lesqa7tl-
JACUSTOMER-lesqa7tl-
39 satisfied customers
I am doing MRVP with catalase test on e. coli and staphylocococcus
I am doing MRVP with catalase test on e. coli and staphylocococcus epidermidis. My results were e.coli and staphylococcus epidermidis were both MR+ & VP- and catalase negative. If they are not the exp… read more
JACUSTOMER-lesqa7tl-
JACUSTOMER-lesqa7tl-
39 satisfied customers
Im doing a case study of a patient who was admitted to hospital
Im doing a case study of a patient who was admitted to hospital with severe pain in the left calf and discolouration over the surface of the skin of the left lower leg. What could be the possible dise… read more
JACUSTOMER-lesqa7tl-
JACUSTOMER-lesqa7tl-
39 satisfied customers
Disclaimer: Information in questions, answers, and other posts on this site ("Posts") comes from individual users, not JustAnswer; JustAnswer is not responsible for Posts. Posts are for general information, are not intended to substitute for informed professional advice (medical, legal, veterinary, financial, etc.), or to establish a professional-client relationship. The site and services are provided "as is" with no warranty or representations by JustAnswer regarding the qualifications of Experts. To see what credentials have been verified by a third-party service, please click on the "Verified" symbol in some Experts' profiles. JustAnswer is not intended or designed for EMERGENCY questions which should be directed immediately by telephone or in-person to qualified professionals.

Disclaimer: Information in questions, answers, and other posts on this site ("Posts") comes from individual users, not JustAnswer; JustAnswer is not responsible for Posts. Posts are for general information, are not intended to substitute for informed professional advice (medical, legal, veterinary, financial, etc.), or to establish a professional-client relationship. The site and services are provided "as is" with no warranty or representations by JustAnswer regarding the qualifications of Experts. To see what credentials have been verified by a third-party service, please click on the "Verified" symbol in some Experts' profiles. JustAnswer is not intended or designed for EMERGENCY questions which should be directed immediately by telephone or in-person to qualified professionals.

Show MoreShow Less

Ask Your Question

x