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HelloThis is Samuel and I will discuss this and provide you information in this regard.Under NY law, there is a Presumption of Paternity, when the Mother is married. That means the husband is presumed to be the biological Father. And it does not matter if you were separated. However, if you can prove that there was no sexual interaction during the separation, then if she proceeds with a child support request then you can file for a Paternity test.
Here is a link to the NY code in this regard.