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There are no specific guidelines to increase the dose of Thyroxine. Thus to achieve control, physicians go by;
1) TSH levels.
2) Previous response to thyroxine on symptoms and thyroid function tests.
3) Free T3 and free T4 values.
I agree that sudden doubling the dose may be towards higher side.
The thyroid antibodies are done; in people who do not respond adequately to the thyroxine for very long time, symptoms keep on persisting, have family history of thyroid disease or there is goiter.
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The stopping of Thyroxine for 2 weeks can affect the result; though not so substantially but many time significantly.
Brands usually should not make a difference, but this can be a factor.
But chance of stopping the medicine would be more likely to affect TSH. So TSh should be repeated after taking it normally for at least 8 weeks.
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