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Hello, this is Dr. Jess. I see you have a question about whether or not to breed your dog or whether you should get the progesterone level measured?
Normally what is recommended is that you record the first day that your bitch shows signs of proestrus, which would be bloody vaginal discharge, swelling of the vulva, and male dogs being interested in her. On day 5 or 6 of proestrus, start "teasing" her with a male dog, and repeat this every 2 to 3 days to determine when her first day of standing heat is. Breed her on the first day of standing heat and then every other day during the period she will accept the male. It may not be too late to test her progesterone level, the issue is once it gets over a certain level, it isn't of value in determining if she has ovulated yet. If you measure the level, and it is over 5, then it cannot be used to determine if she has ovulated. To determine if ovulation is occurring, you have to have a baseline progesterone level measured, followed by a subsequent higher progesterone level to show that ovulation is occurring.
So you could try to measure the progesterone now, if it is 5 or over, it isn't going to be of value to you. If it is less than 2, then you should remeasure in 2 days, and keep measuring it every 2 days until it jumps to over 2, then you will know she is going to ovulate and should breed her every other day starting ASAP.
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