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=) Thank you so much
Yes, I can see the bites in the cells
Heinz bodies are formed by damage to the hemoglobin component molecules, usually through oxidant damage, or from an inherited mutation (i.e. change of an internal amino acid residue). As a result, an electron from the hemoglobin is transferred to an oxygen molecule, which creates a reactive oxygen species (ROS) that can cause severe cell damage leading to premature cell lysis. Damaged cells are cleared by macrophages in the spleen, where the precipitate and damaged membrane are removed, leading to characteristic "bite cells". The denaturing process is irreversible and the continual elimination of damaged cells leads to Heinz body anemia.
There are several pathways leading to the hemoglobin damage.
Wow, didn't thought it was caused by the onions. =) But I learned something new about veterinary medicine and what it causes and condition of a pet it could lead to. Thank you so much sir
Right, isn't that weird that onions do that? I remember when I first learned that, and I thought it was weird as well. This actually does happen in practice ever so often too.Ok .. here's a link to some info on Diff Quick : (just click here) This one says about 30 seconds for each step, but I have also done 15 dips in the first step, 16 dips in the second, 17 in the last one. But, that's just what I was taught in school. *shrug*Essentially, three steps.1. alcohol fixative2. eosinophilic stain (dyes stuff red like red blood cells, some bacteria (negatives))3. basophilic stain (it's the purple stuff) ... it's a counter stain. What that means is that it dyes most of the other stuff on the slide purple (or kind of a bluish purple). This helps the eosinophilc stained items stand out, and they are more easily seen. Gram positives like staph bacteria show up as little purple circles.That is probably not a very good explaination. So, you might need to check the notes for exact types of stain, dyes, etc that are in the ingredients of the Wright's stain. Remember Diff Quick is just one type of commercial Wright's stain. It's a little faster and better than the old fashioned original Wright's stain... so, you might need to know that.This is probably a better explaination of Wright's Stain : "
The traditional Wrights Stain dates from the early 1890's. The original Wrights Stain was an alcoholic solution of methylene blue and eosin Y. Since then there have been many modificiations, most involving partial oxidative demethylation of the methylene blue to improve polychroming. Modern day samples of the dye usually contain mixtures of methylene blue, azure A, thionin and eosin Y. They also contain some amount of giemsa stain.
The traditional stain is diluted 1:1 with giordano buffer before use. One Step Wrights Stain contains the buffer already dissolved in the stain. The slides are stained in the undiluted stain and differentiated by decolorizing in purified water."
Thank you so much Sir for all help! I"ll really do appreciate your time and your opinions. Thanks I've learned a lot more about veterinary medicine. Thank you one more time and have a great day!