Accepted Answer

I presume you are asking about opening a museum in Alberta as you are located there?

If so, the Alberta Museums Association (or Museums Alberta) has information on how to open a museum including an excellent downloadable pdf booklet entitled " Thinking about starting a museum?". Please check out their website and contact them for further information :

http://www.museumsalberta.ab.ca/starting.html

Museums Alberta
9859 - 103 Street
Edmonton, AB
T5K 0X9
Phone: XXX-XXX-XXXX
Fax: XXX-XXX-XXXX

Email: Info@museumsalberta.ab.ca

The Alberta Museums Association is a non-profit society formed by individuals, both museum professionals and volunteers, to promote the development of Alberta's museums and galleries. They do not mention licenses at all but they do offer grant applications and advisory services that you may be interested in :

http://www.museumsalberta.ab.ca/advisor.html

The government department that is involved with museums is the Alberta Government Community Development :

http://www.cd.gov.ab.ca/index.asp