Your timing may be off as dogs only mate specific times in the heat cycle or your male may not be able to 'reach'.
Dogs are usually in heat for about 3 weeks. The first week they have a red discharge, then the second a clear or straw colored discharge, then almost no discharge the last week. They usually are willing to breed sometime in the middle of their heat cycle usually in the range of days 9-14 of their heat season. Dogs are individuals so you have to see when the female is agreeable as there is no one specific day for sure. Heat cycles can vary in length for an individual also.
One sign of her being ready would be for her to 'flag' or roll her tail to one side when touched on her hind quarters.
You may want a vet to do the artificial insemination for you - this site has a listing of repro vets who may help if your vet will not
http://www.whelpwise.com/testing/veterinarian-links.html
and this lists more
http://www.therio.org/search.cfm
This site shows how collection is done
http://www.vetmed.lsu.edu/eiltslotus/theriogenology-5361/dog--white_2.htm
and here is a video on the topic
http://www.youtube.com/watch?v=qxXlniylh5g
Hope this helps you!
Dog Expert:Rescue, Train,Breed,Care
30+ yrs dog home vet care & nursing, rescue, behavior&training, responsible show breeding, genetics
I gave you some instructions and a video - if you didn't see that please let me know!
The key is to make sure you are using sterile instruments and the right equipment which is why it can be easier using a vet for immediate AI
http://leerburg.com/1074.htm
http://www.pinnaclepetsupply.com/ai-kits.htm
thanks for your above accept!