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Dr. Mo
Dr. Mo, Board Certified MD
Category: Medical
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Experience:  Urgent Care Physician
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How can you test a urine sample for pH, glucose, and protein

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How can you test a urine sample for pH, glucose, and protein without using a test strip? What would be the reagents, procedures, and results interpretations?
JA: Is this a time sensitive issue? What type of specimen are they testing?
Customer: I don't assume they're time sensitive. Routine urine sample
JA: Anything else in your medical history you think the doctor should know?
Customer: No, this is actually a question for a class

Please give me a moment to reply.

Methyl red and bromothymol blue are used for the pH testing.

Glucose oxidase is what's used for the glucose detection which undergoes an enzyme reaction.

Protein is measured usually through the reagent called tetrabromophenol.

These are pH regulated and change pH based on the protein content (this doesn't have anything to do with the other pH test).

The results and interpretations couldn't be done unless you had some control chemicals to use to calibrate the test with.

For example, if you knew that one sample had a certain amount of glucose and another sample a higher glucose then you could calibrate your urine test by knowing how the those reagents react based on the level of color change.

Please let me know if the information helped or if there is anything I can clarify further.
Customer: replied 8 days ago.
What is the standard testing procedure for tetrabromophenol?

Tetrabromophenol is simply called Bromophenol blue.

I am not quite sure what you mean by standard testing procedure. It reacts to the acidity and basicity of whatever compound it comes in contact with. The reaction has to do with the hydronium ion or hydrogen ions which react with this chemical to cause the color change from the various shades of yellow to blue.

One can take a known pH solution and react it with the TBP compound and find out what color it takes on. This would standardize the color and a chart can be created.

Please let me know if that helps.

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