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In what US state are you? Have you involved the authorities?
I do not know what US state you are in but in general, there are several things that you can do to eliminate the problem. If the signs and fences don't work, you can contact a lawyer and have him send a cease and desist letter to your neighbors. This is likely similar to what you have already sent you, in that its purpose is to put him on notice that you won't tolerate his trespassing any more. However, when it comes certified mail from a lawyer on legal letterhead, he'll be more apt to read it and abide by its terms than if you send it yourself.
From there, you can see, to have him prosecuted for his trespassing on to your land. Or you could sue him in the civil courts and ask for injunctive relief.
In some states, you may also be able to just get a civil restraining order against him for the trespassing which would then, once he is served with the order, subject him to criminal charges if he fails to abide by the terms of the order. If you let me know what US state you are in, I can check to see if your state would afford you that remedy.
Just checking in to see if you need more help or any clarification of my answer. If so, please reply here on this question thread.