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Yes, you need to file an eviction. Florida does not allow landlords to utilize self-help. Under Florida law, you will first serve a "Three Day Notice" of eviction and past due rent. If the tenants do not pay then you will file a Complaint in County Court for eviction and have the document served on the tenants. You may also include a count for past due rent. Once you receive a judgment in your favor then a "Writ of Possession" will be issued by the Court. Once issued, you wil pay a fee for the Sheriff's office to "execute" on the writ and remove any and all tenants living on the property.
I would recommend consulting a local attorney to protect your interests and rights.