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If you have already sent your cease and desist letter and they are continuing to violate your trademark rights, you would have to file a complaint for trademark infringement in the US District Court and together with that complaint you have to file a motion for preliminary injunction to obtain a court order to them to cease using your mark. If the trademark belongs to a business, the business must file suit and a business (corporation, S-Corp or LLC or Partnership) must be represented by an attorney and an owner cannot represent the business in a legal matter as it would be considered an unauthorized practice of law since a business is considered a separate legal entity that can sue and be sued. If you personally own the trademark under your personal name, you can file the suit and motion for the preliminary injunction on your own. To file this in the online US District Court system, you have to arrange with the clerk of the court to have an online account and if not you have to file it in person and then you have to serve the other party using a process server with a copy of the complaint, motion and summons.
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