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It's a simple routine matter for a judge to order Paternity Testing where the Paternity of a child is established, so it's very odd that the magistrate would force your brother to go out and find statutes to cite to prove that he's entitled to it. Public policy supports a child having a relationship with both parents and being supported by both parents.

With that said, the relevant statute is Fl. Stat., Section 742.12.
In any proceeding to establish paternity, the court may, upon request of a party providing a sworn statement or written declaration as provided by s. 92.525(2) alleging paternity and setting forth facts establishing a reasonable possibility of the requisite sexual contact between the parties or providing a sworn statement or written declaration denying paternity and setting forth facts establishing a reasonable possibility of the nonexistence of sexual contact between the parties, require the child, mother, and alleged fathers to submit to scientific tests that are generally acceptable within the scientific community to show a probability of paternity. The court shall direct that the tests be conducted by a qualified technical laboratory.

If the man listed on the birth certificate signed a voluntary acknowledgment of paternity, your brother had 60 days to contest it. Still, Section 742.10 allows him to dispute that man's paternity based on a material mistake of fact or fraud, which covers both situations where the other man didn't know, or where he intentionally lied.