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No, the new marriage will not "invalidate" the old will, but state law does allow for an "elective share" for his spouse. Marriage does alter a will to the extent that North Carolina law provides an “elective share” of a deceased person’s estate to his or her spouse. This share is equal to 1/3 of the estate and is guaranteed to the spouse regardless of what the will might say.
So while she can't get everything, she can claim up to 1/3 of the estate.
The marriage does not invalidate that will leaving property to you.
But it does give her the right to claim this share.
Now it's possible to challenge the marriage post mortem based on fraud or capacity.
To be honest, this would be difficult, but in certain situations if you could show that your step father did not have mental capacities to enter into marriage, then that marriage could be "annulled" after the fact.