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Once you resigned you have harmed any case you may have had. From what you are describing, it sounds like you potentially could have been discriminated against based on your gender, but you did not really give us any specific evidence above showing that. As far as discrimination under the FMLA, if you had not been there at least 12 months, FMLA would not apply, so you could not claim discrimination or retaliation under that law. Thus, the only claim you could have based on what you are saying is you think is sex discrimination, but you need to point out some evidence as to how you were treated differently only based on your sex. If you have that evidence, then your next step is filing a complaint with the EEOC within 300 days of your termination alleging constructive discharge as the reason you resigned rather than be fired and they must investigate and grant you a right to sue letter before you could go to court.