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In and of itself, probably not. If they take action on this and decide to lay you off, then you would have an argument that they treated you differently based on your disability, in violation of the ADA.
Simply reviewing a job description (written for a subsequent hire) is not problematic. Asking questions is not a problem. Perhaps they were giving you an opportunity to raise and accommodations you will need. That said, if they let you go and they have used seniority for every other lay off... yes, they would be illegally discriminating against you most likely.
My department director knows that I cannot drive. The job description states I would be responsible for attending meeting out of the facility and visiting the 7 other sites associated with the facility. It just seems to me that this is wrong. I feel like I'm being pushed out. Can they do this?