Thank you for writing back. I am sorry about this problem. Are you sure that this memory card really has your old pictures? I am asking because if you use Recuva with deep scan, you should always see deleted files that you can recover even if not all of your deleted files are recoverable.
The 2 software that I gave you are the best free tools available out there. If these tools did not recover your data, then please try to use the Free tool from Easeus. If the free tool won't recover your data, then your next option is to use the Pro or paid version. (When using the Paid version, just get a free rial first and see if the trial version recovers your images).
To get the FREE tool, click on this link:http://www.easeus.com/datarecoverywizard/free-data-recovery-software.htm
Now, if no tools are able to recover your overwritten data, I don't suggest formatting the card since it will destroy good data from the card. However, you can backup the files and then format the memory card, and then perform the recovery or deep scan to test for deleted files.
Do you know why this happened? Basically, I experienced this problem personally with my Canon camera. The cause of the problem is that, when you insert the memory card to the computer card slot, Windows (your OS) will "write" to the card without you knowing it, or without you initiating it. As a result, the files will no longer be recognized by the camera.
The solution to this is to LOCK the memory card before inserting to the computer card slot. This will allow you to access the pictures but you cannot save any data to the card from the computer. This will also prevent Windows from writing to your card that will cause this problem that you are having now.
To LOCK the memory card, please check for a small sliding switch in the side of the card and push it to the LOCKED location to lock it. When you put the card back to the camera, that is the time to UNLOCK the card so that captured pictures will be saved to the card.
Here is a sample SD Card lock:
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