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Bactbob
Bactbob, Public Health Laboratory Director
Category: General
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Experience:  20 years as a Public Health Microbiologist/Director
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Fermentation profile for S.epidermidis and S.cervisiae using

Resolved Question:

Fermentation profile for S.epidermidis and S.cervisiae using Phenol Red test (glucose,fructose,mannitol)


How soon can I get an answer?
Submitted: 4 years ago.
Category: Microbiology
Expert:  replied 4 years ago.

Bactbob :

Pretty fast, just let me look it up to make sure I'm giving you accurate information

Bactbob :

Staphyloccuss epidermidis will produce acid from glucose, lactose, sucrose, mannitoland glycerol. No acid from raffinose, salicin or inulin.
Acid production is Phenol red turning yellow

Bactbob :

Still working on the yeast fermentation

Customer:

what about gas?

Bactbob :

saccharomyces cerevisiae: glucose fructose Postive mannitol Negative

Bactbob :

Saccharomyces will produce gas, staph will not..

Bactbob :

Still checking on fructose for Staph.. That is not normally tested in the identification so I'll have to check some more resources

Bactbob :

Staph will produce gas with lactose, but not the other sugars

Bactbob :

It looks like Staph will ferment fructose, but I haven't ever run that sugar against Staph, so can't be 100% certain

Customer:

Are you sure s. epidermidis ferments mannitol?

Bactbob :

Found a reference in Bergey's manual. Staph will ferment fructose

Bactbob :

Let me double check and give you the reference

Bactbob :

That link says negative for mannitol, but other reference tests say differently..I'll give you those too

Bactbob :

sorry, my mistake, I was looking at a research text on a variant of S. epidermidis

Bactbob :

http://www.archive.org/stream/bergeysmanualofd1957amer/bergeysmanualofd1957amer_djvu.txt
Distinctive characters: Coagulase-nega- tive. Ferments glucose but not mannitol under anaerobic conditions.

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